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National Repository of Grey Literature

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Zero Ionic Strength Corrections of pKA Constants and Electrophoretic Mobilities of Weak Electrolytes Lorinčíková, Kateřina ; Gaš, Bohuslav (advisor) ; Sobotníková, Jana (referee) The dependencies of the electrophoretic mobility of histidine on pH were measured in two sets of buffers at 20mM ionic strength. The parameters, which were obtained from these curves, were subsequently corrected to zero ionic strength using an older approach, as well as using the newly developed program AnglerFish. The effect of the ionic atmosphere on the pKA and mobility is shown, as well as the shape of the relevant corrections, which allow the determination of the pKA and mobility at infinite dilution. A new compound, imatinib, which is a quadruple positively charged nitrogenous heterocyclic compound, was also measured, and its pKA constants and mobilities were likewise determined. Key Words capillary zone electrophoresis, ionic strength, limiting mobility, pKA, zero ionic strength corrections Detailed record

Determination of dissociation constants and limiting mobilities of some ampholytes Maliňáková, Lucie ; Riesová, Martina (advisor) ; Beneš, Martin (referee) Capillary zone electrophoresis is a simple and automated method which enables simultaneous determination of the dissociation constants and limiting ionic mobilities of the analyte from one series of experiments. The small consumption of the sample is main advantage of this method. The dependence of the effective mobilities of 7 selected ampholytes on pH in the range of 1.6 to 6.8 was measured. A set of simple buffers with constant ionic strength and satisfactory buffering capacity was designed using computer program PeakMaster. The ampholytes were detected by contactless conductivity detector. Dynamic capillary coating was used to minimize interactions of analytes with silanol groups on the inner wall of the capillary. Experimentally obtained effective mobilities were fitted with the suitable function. Actual mobilities and the mixed dissociation constants corresponding to the actual ionic strength were parameters of the regression function. Thermodynamic dissociation constants and limiting ionic mobility of ampholytes were calculated using the extended Debye-Hückel law with the linear member and the Onsager law. Key words: capillary zone electrophoresis, dissociation constant, limiting mobility, ampholytes Detailed record

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